Trehalose is a potent PCR enhancer: lowering of DNA melting temperature and thermal stabilization of taq polymerase by the disaccharide trehalose.
نویسندگان
چکیده
References 1. Ostermeier C, Iwata S, Michel H. Cytochrome c oxidase. Curr Opin Struct Biol 1996;6:460–6. 2. Sullivan JL, Ochs HD. Copper deficiency and the immune system. Lancet 1978;2:686. 3. Vyas D, Chandra RK. Thymic factor activity, lymphocyte stimulation response and antibody producing cells in copper deficiency. Nutr Res 1983;3:343–50. 4. Araya M, Koletzko B, Uauy R. Copper deficiency and excess in infancy: developing a research agenda. J Pediatr Gastroenterol Nutr 2003;37: 422–9. 5. Widdowson E. Trace elements in fetal and early postnatal development. Proc Nutr Soc 1974;33:275–84. 6. Al-Rashid RA, Sprangler J. Neonatal copper deficiency. N Engl J Med 1971;285:841–3. 7. Ashkenazi AS, Levin S, Djaldetti M, Fisher E, Benvestini D. The syndrome of neonatal copper deficiency. Pediatrics 1973;52:523–33. 8. William DM. Copper deficiency in humans. Semin Hematol 1983;20:118– 28. 9. Cizewski-Culuta V, Gitlin JD. Disorders of copper transport. In: Scriver CR, Beaudet AL, Sly WS, eds. Metabolic and molecular bases of inherited disease, 8th ed. New York: McGraw-Hill, 2000:3105–28. 10. Hannam S, McDonnell M, Rennie IM. Investigation of prolonged neonatal jaundice. Acta Pediatr 2000;89:694–7. 11. Maisel JM. Jaundice in neonatology. In: Avery GB, Fletcher MA, McDonald MG, eds. Pathophysiology and management of the newborn, 4th ed. Philadelphia: JB Lippincott, 1994:630–60. 12. Henry RJ, Chiamoris N, Jacobs SL, Seaglove M. Determination of ceruloplasmin oxidase activity in serum. Proc Soc Exp Med Biol 1960;104:620–4. 13. Terres-Martos C, Navarro-Alarcon M, Martin-Lagos F, Lopez-Carcia de la Serrana H, Lopez Martinez MC. Determination of Cu levels in serum of healthy subjects by atomic absorption spectrometry. Sci Total Environ 1997;198:97–103. 14. Bremer J. Manifestations of copper excess. Am J Clin Nutr 1998;67(Suppl): 1069–73. 15. Fuentealba C, Aburto EM. Animal models of copper-associated liver disease. Comp Hepatol 2003;2:1–12. 16. Valaes T, Karaklis A, Stavrakakis D, Bavela-Stavrakakis K, Perakis A, Doxiadis SP. Incidence and mechanism of neonatal jaundice related to glucose-6-phosphate dehydrogenase deficiency. Pediatr Res 1969;3:448– 58. 17. Kaplan M, Beutler E, Vreman HJ, Hammerman C, Levy-Lahad E, Renbau P, et al. Neonatal hyperbilirubinemia in G6PD deficient heterozygotes. Pediatrics 1999;104:68–74. 18. Rossipal E, Krachler M, Li F, Micetic-Turk D. Investigation of the transport of trace elements across barriers in humans: studies of placental and mammary transfer. Acta Paediatr 2000;89:1190–5. 19. Chen BH, Tsai JL, Tsai LY, Chao MC. Comparison of serum copper, magnesium, zinc and calcium levels between G6PD deficient and normal Chinese adults. Kaohsiung J Med Sci 1999;15:646–50. 20. Schilsky ML, Scheinberg IH, Sternlieb I. Liver transplantation for Wilson disease. Indications and outcome. Hepatology 1994;19:583–7. 21. Strand S, Hofmann WS, Grambihler A, Hug H, Volkmann M, Otto G, et al. Hepatic failure and liver cell damage in acute Wilson’s disease involve CD 95 (Apo-I/Fas) mediated apoptosis. Nat Med 1998;4:588–93. 22. Suzuki KT, Shiobara Y, Tachibana A, Ogra Y, Matsumoto K. Copper increases in both plasma and red cells at the onset of acute hepatitis. Res Commun Mol Pathol Pharmacol 1999;103:189–94. 23. Sokol RJ. Antioxidant defenses in metal induced liver damage. Semin Liver Dis 1996;16:39–46. 24. Fridovich J. Superoxide anion radical superoxide dismutases and related matters. J Biol Chem 1997;272:515–7. 25. Sokol RJ, McKim JM, Devereau MW. -Tocopherol ameliorates oxidant injury of isolated copper overloaded rat hepatocytes. Am J Clin Nutr 1998; 67(Suppl):1012–6. 26. Fridovich J. Superoxide radical and superoxide dismutases. Annu Rev Biochem 1995;64:97–112. 27. Van Wassenaer-van Hall HN. Neuroimaging in Wilson’s disease. Metab Brain Dis 1996;12:1–19.
منابع مشابه
1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer
BACKGROUND Quantitative real-time PCR (qPCR) is becoming increasingly important for DNA genotyping and gene expression analysis. For continuous monitoring of the production of PCR amplicons DNA-intercalating dyes are widely used. Recently, we have introduced a new qPCR mix which showed improved amplification of medium-size genomic DNA fragments in the presence of DNA dye SYBR green I (SGI). In ...
متن کاملTrehalose Plays Role in cDNA Research
The advent of thermostable enzymes has led to great advances in molecular biology, such as the development of PCR and ligase chain reaction. However, isolation of naturally thermostable enzymes has been restricted to those existing in thermophylic bacteria. Here, we show that the disaccharide trehalose enables enzymes to maintain their normal activity (thermostabilization) or even to increase a...
متن کاملThermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA (chaperonin-like moleculesypolyolsyenzyme thermostabilityyreverse transcriptase)
The advent of thermostable enzymes has led to great advances in molecular biology, such as the development of PCR and ligase chain reaction. However, isolation of naturally thermostable enzymes has been restricted to those existing in thermophylic bacteria. Here, we show that the disaccharide trehalose enables enzymes to maintain their normal activity (thermostabilization) or even to increase a...
متن کاملFunctional analysis of glycin rich- RNA binding protein, a suppressor of trehalose-6-phosphate mediating growth arrest in Arabidopsis thaliana
Metabolism of the alpha-1,1 glucose disaccharide, trehalose, is indispensable in plants. In the Murashigeand Skoog (MS) medium, trehalose inhibits plant growth and allocation of carbon to roots. A suppressorof trehalose-6-phosphate (T6P) mediated growth arrest, GR-RBP2, is characterized in more detail.Phylogenetic analysis revealed that GR-RBP2 is a protein of likely prokaryot...
متن کاملThermostabilized chondroitinase ABC Promotes Neuroprotection after Contusion Spinal Cord Injury
Background: Chondroitinase ABC (cABC), due to its loosening impact on the extracellular matrix scaffold, has been used to enhance regeneration of injured axonal tracts after spinal cord injury (SCI). However, cABC thermal instability at physiological temperature has limited its clinical application. The disaccharide trehalose has been shown to increase the stability of cABC in body temperature....
متن کاملEffect of Trehalose on the Expression of Heat Shock Protein 70 Gene in PC12 Cells Treated with Hydrogen Peroxide
Background and purpose: Oxidative stress is implicated in the pathogenesis of various diseases, including neurological disorders. In such stressful conditions, heat shock protein 70 (HSP70) protects cells, thus its pharmacological induction can be protective. The disaccharide trehalose exhibits various beneficial effects, including antioxidative effect. However, its impact on HSP70 is not obvio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 50 7 شماره
صفحات -
تاریخ انتشار 2004